Educational lectures

Confirmed Speakers for Invited Educational Lectures / Educational Objectives

 

Session: Spin Probes, Spin Traps, Spin Labels for EPR and DNP

 

Benoit Driesschaert (Morgantown, WV, USA)

Probing the local environment with stable triarylmethyl (TAM) radicals: what can be measured and best practices

Educational objectives:

  • Understanding the rational design of TAM radicals leading to their high stability, functional sensitivity, and in vivo toxicity profile.

  • TAM spin probes for the measurement of oxygen, pH, microviscosity, inorganic phosphate, redox, and bio-thiol concentration. Understanding which probes to use, best practices, and confounding factors.

  • Understanding which modes of delivery are available: Intra-venous, retro-orbital, intra-peritoneal, or intra-tissue injection, and how does the delivery mode influence the reported parameter?

 

Adam Sikora (Lodz, Poland)

Detection of peroxynitrite with the use of boronate probes - focus on free radical transient species

 Educational objectives:

  • Basics of the chemistry of peroxynitrite

  • The concept of boronate redox probes

  • Reaction of boronate probes with peroxynitrite - formation of radical transient species

  • Spin trapping - towards the understanding of reaction mechanism

  • Looking for peroxynitrite fingerprints

 

Session: Macromolecules and Metal Proteins

 

Janet Lovett (St Andrews, UK)

The use of DEER and RIDME for measuring nanometre distances in biomacromolecules

Educational objectives:

  • To gain an understanding that EPR can measure nanometre scale distances and distributions between pairs of paramagnetic centres via their dipolar coupling.

  • To appreciate that the paramagnetic centres can be diverse and either intrinsic to the protein or positioned site-specifically using a variety of spin labelling methods, and that these can have properties that are tunable for the experiment at hand.

  • To realise the variety of pulsed EPR measurements available and to gain a deeper understanding of DEER and RIDME pulse experiments, including how data are analysed.

  • To see some recent applications of DEER and RIDME to diverse biological systems.

 

Ines Garcia Rubio (Zaragoza, Spain)

What can EPR tell about heme proteins?

Educational objectives:

  • CW signatures of the different spin states of Fe in hemeproteins

  • Sample preparation. Tips and pitfals.

  • Hyperfine structure. Mesurement and interpretation

 

Oxidative stress

 

Michael Davies (Copenhagen, Denmark)

Non ambiguous characterization of reactive free radicals with EPR 

 Educational objectives:

  • Advantages and disadvantages of different methods to detect reactive radicals in biological systems

  • Trapping of radicals with different spin traps

  • Methods to provide definitive assignments

  • Assessment of radical versus non-radical pathways: use of complementary (orthogonal) approaches

 

 

Technology and Instrumentation

 

Gunnar Jeschke (Zurich, Switzerland)

Instrumentation for and spin dynamics during frequency-swept pulses in EPR

Educational objectives:

  • How to achieve very broadband excitation of electron spins

  • How to achieve high spectral purity of excitation waveforms

  • Behavior of a single transition upon passage

  • Interference effects for connected transitions and how to take advantage of them

  • Dressed-spin excitation by phase-modulated irradiation

 

Mark Tseytlin (Morgantown, WV, USA)

Rapid Scan EPR: principles, instrumentation, and applications.

Educational objectives:

  • A brief introduction to the evolution of CW EPR

  • Digital EPR instrumentation

  • RS methodology and practical aspects of this technique, such as what types of probes and experiments it will be beneficial for.  

  • 4D and 5D RS EPR imaging and applications

 

 

 
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